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42-365 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-365 (3.75ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-365 (0.3ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AES Antibody

CATALOG NUMBER: 42-365

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: SHLPQQLKFTTSD-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 29.1kDa according to NP_945320.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Colon. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_945320.1; NP_001121.2; NP_945321.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Positive Control 2:
Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AES
Additional Names:
AES, amino-terminal enhancer of split, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, amino enhancer of split, gp130-associated protein GAM
Protein Accession Number:
NP_945320.1,NP_001121.2,NP_945321.1
Protein Gi Number:
39812019
Ncbi Gene Id Number:
166

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-365

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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