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Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of AFAP1 in Hela cell lysate with AFAP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

AFAP1 Antibody

CATALOG NUMBER: 6557

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (78%)
Immunogen:
AFAP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AFAP1.
The immunogen is located within amino acids 30 - 80 of AFAP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
AFAP1 antibody can be used for detection of AFAP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
Monomer and homomultimer of AFAP1 are known to exist; AFAP1 antibody is predicted to not cross-react with other AFAP family members.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
AFAP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AFAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AFAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AFAP1
Additional Names:
AFAP1 Antibody: AFAP, AFAP110, AFAP-110, AFAP, Actin filament-associated protein 1, 110 kDa actin filament-associated protein
Protein Accession Number:
NP_940997
Protein Gi Number:
197382472
Ncbi Gene Id Number:
60312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AFAP1 Antibody: The actin filament-associated protein AFAP1 (AFAP-110) is an actin cross-linking protein first identified as a substrate of the viral oncogene v-Src. AFAP1 has a fundamental role in actin cytoskeleton arrangement. It contains a carboxyterminal actin-binding domain that directly binds to F-actin and serves as an adaptor protein in the regulation of SRC and PKC signal transduction by several functional domains, including 2 pleckstrin homology (PH) domains, a Src homology 3-binding (SH3-binding) motif, and several SH2-binding motifs. It is overexpressed in prostate carcinoma and contributes to tumor growth by regulating cell-matrix adhesions and migration in the cancer cells. AFAP1 represent a possible therapeutic target for controlling tumorigenesis and metastasis.
Background Reference 1:
Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
Background Reference 2:
Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
Background Reference 3:
Baisden JM, Qian Y, Zot HM, et al. The actin filament-associated protein AFAP-110 is an adaptor protein that modulates changes in actin filament integrity. Oncogene 2001; 20:6435-47.
Background Reference 4:
Zhang J, Park SI, Artime MC, et al. AFAP-110 is overexpressed in prostate cancer and contributes to tumorigenic growth by regulating focal contacts. J. Clin. Invest. 2007; 117:2962-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6557

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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