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Western blot analysis of extracts of various cell lines, using AFF4 antibody (19-432) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using AFF4 antibody (19-432) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 30s.

AFF4 Antibody

CATALOG NUMBER: 19-432

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human AFF4 (NP_055238.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
U-87MG
Positive Control 2:
HeLa
Positive Control 3:
293T
Positive Control 4:
LO2
Positive Control 5:
MCF7
Positive Control 6:
Mouse pancreas
Predicted Molecular Weight:
Observed: 150kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AFF4
Additional Names:
AF5Q31, CHOPS, MCEF, AF4/FMR2 family member 4, ALL1-fused gene from chromosome 5q31 protein, major CDK9 elongation factor-associated protein
Ncbi Gene Id Number:
27125
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-432

List Size:
100 uL

List Price:

$436.00

Datasheet
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