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Western blot analysis of extracts of various cell lines, using AFG3L2 antibody (16-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using AFG3L2 antibody (16-029) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using AFG3L2 antibody (16-029) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

AFG3L2 Antibody

CATALOG NUMBER: 16-029

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2 (NP_006787.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
293T
Positive Control 3:
HeLa
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 89kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AFG3L2
Additional Names:
AFG3L2, FLJ25993, SCA28, SPAX5
Ncbi Gene Id Number:
10939
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-029

List Size:
100 uL

List Price:

$436.00

Datasheet
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