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Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 μg/ml.
Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 μg/mL.
Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 μg/mL.
Immunofluorescence of AFP in mouse liver tissue with AFP antibody at 20 μg/mL.<br><br>Red: AFP Antibody (5869) <br> Blue: DAPI staining
Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 μg/mL and (B) 2 μg/mL.
Trial Size

AFP Antibody

CATALOG NUMBER: 5869

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (91%), Sheep: (83%), Bovine: (83%)
Immunogen:
AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP.
The immunogen is located within amino acids 340 - 390 of AFP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Purification:
AFP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AFP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AFP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AFP
Additional Names:
AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
Protein Accession Number:
NP_001125
Protein Gi Number:
4501989
Ncbi Gene Id Number:
174
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AFP Antibody: Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal development. In adults, AFP expression is often associated with hepatoma or teratoma. However, hereditary persistance of AFP may also be found in individuals with no obvious pathology. AFP is thought to be the fetal counterpart of serum albumin, and the AFP and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. AFP is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. AFP levels in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
Background Reference 1:
Lafuste P, Robert B, Mondon F, et al. Alpha-fetoprotein gene expression in early and full-term human trophoblast. Placenta2002; 23:600-12.
Background Reference 2:
Walhof CM, Van Sonderen L, Voute PA, et al. Half-life of alpha-fetoprotein in patients with a teratoma, endodermal sinus tumor, or hepatoblastoma. Pediatr. Hematol. Oncol.1988; 5:217-27.
Background Reference 3:
Nishio H, Heiskanen M, Palotie A, et al. Tandem arrangement of the human serum albumin multigene family in the sub-centromeric region of 4q: evolution and chromosomal direction of transcription. J. Mol. Biol.1996; 259:113-9.
Background Reference 4:
Deutsch HF. Chemistry and biology of alpha-fetoprotein. Adv. Cancer Res.1991; 56:253-312

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5869

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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