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Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
AFTPH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

AFTPH Antibody

CATALOG NUMBER: 62-045

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This AFTPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 844-871 amino acids from the C-terminal region of human AFTPH.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
102 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AFTPH
Additional Names:
Aftiphilin, AFTPH, AFTH
Protein Accession Number:
Q6ULP2
Protein Gi Number:
62286617
Ncbi Gene Id Number:
54812
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AFTPH may play a role in membrane trafficking.
Background Reference 1:
Lui-Roberts, W.W., et al. Mol. Biol. Cell 19(12):5072-5081(2008)
Background Reference 2:
Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Background Reference 3:
Hirst, J., et al. Mol. Biol. Cell 16(5):2554-2565(2005)
Background Reference 4:
Burman, J.L., et al. FEBS Lett. 579(10):2177-2184(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-045

List Size:
400 ul

List Price:

$495.00

Datasheet
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