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Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF7 cells using AFX (Phospho-Ser197).
Western blot analysis of lysed extracts from 293 cells untreated or treated with serum using AFX (Phospho-Ser197).

AFX (phospho Ser197) Antibody

CATALOG NUMBER: 79-168

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of AFX only when phosphorylated at serine 197.
Predicted Molecular Weight:
65 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
FOXO4
Additional Names:
AFX, AFX1, MLLT7, EIF2A, AFX, FOXO4, Afxh
Protein Accession Number:
NP_001164402.1
Protein Gi Number:
283436083
Ncbi Gene Id Number:
4303
Background:
Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
Background Reference 1:
Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21 (10): 3534-46. Kops GJ, et al. (1999) Nature; 398 (6728): 630-4.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-168

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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