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Western blot analysis of AGER Antibody in mouse lung tissue lysates (35ug/lane)
AGER Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AGER Antibody in mouse lung tissue lysates (35ug/lane)

AGER Antibody

CATALOG NUMBER: 61-687

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This AGER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human AGER.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGER
Additional Names:
Advanced glycosylation end product-specific receptor, Receptor for advanced glycosylation end products, AGER, RAGE
Protein Accession Number:
Q15109
Protein Gi Number:
2497317
Ncbi Gene Id Number:
177
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AGER encodes a member of the immunoglobulin superfamily of cell surface molecules. It is a receptor for various molecules, including the amyloidogenic form of serum amyloid A, amyloid-beta protein, members of the S100/calgranulin superfamily and advanced glycation end products. The gene lies within the major histocompatibility complex (MHC) class III region on chromosome 6.
Background Reference 1:
Rojas, A., et al. Carcinogenesis 31(3):334-341(2010)
Background Reference 2:
Engelen, L., et al. J. Hypertens. 28(2):285-293(2010)
Background Reference 3:
Poon, P.Y., et al. Clin. Nephrol. 73(1):44-50(2010)
Background Reference 4:
Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-687

List Size:
400 ul

List Price:

$495.00

Datasheet
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