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Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (22-279) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug AGPAT1 antibody (22-279). Western blot was performed from the immunoprecipitate using AGPAT1 antibody (22-279) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using AGPAT1 antibody (22-279) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

AGPAT1 Antibody

CATALOG NUMBER: 22-279

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-283 of human AGPAT1 (NP_006402.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
SW620
Positive Control 3:
Mouse kidney
Positive Control 4:
Rat kidney
Predicted Molecular Weight:
Observed: 32kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AGPAT1
Additional Names:
1-AGPAT1, G15, LPAAT-alpha, LPAATA, 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha, 1-AGP acyltransferase 1, 1-AGPAT 1, 1-acylglycerol-3-phosphate O-acyltransferase 1 (acetoacetly Coenzyme A thiolase), 1-acylglycerol-3-phosphate O-acyltransferase 1 (lysophosphatidic acid acyltransferase, alpha), epididymis secretory sperm binding protein, lysophosphatidic acid acyltransferase alpha, lysophospholipid acyltransferase
Ncbi Gene Id Number:
10554
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme that converts lysophosphatidic acid (LPA) into phosphatidic acid (PA). LPA and PA are two phospholipids involved in signal transduction and in lipid biosynthesis in cells. This enzyme localizes to the endoplasmic reticulum. This gene is located in the class III region of the human major histocompatibility complex. Alternative splicing results in two transcript variants encoding the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-279

List Size:
100 uL

List Price:

$436.00

Datasheet
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