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Western blot analysis of AGR3 Antibody in MCF-7 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with AGR3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of AGR3 Antibody in MCF-7 cell line lysates (35ug/lane)

AGR3 Antibody

CATALOG NUMBER: 63-940

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AGR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-147 amino acids from the C-terminal region of human AGR3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGR3
Additional Names:
Anterior gradient protein 3 homolog, AG-3, AG3, hAG-3, Breast cancer membrane protein 11, AGR3, BCMP11
Protein Accession Number:
Q8TD06
Protein Gi Number:
66774045
Ncbi Gene Id Number:
155465
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.
Background Reference 1:
Persson, S., et al. Mol. Phylogenet. Evol. 36(3):734-740(2005)
Background Reference 2:
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Background Reference 3:
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Background Reference 4:
Fletcher, G.C., et al. Br. J. Cancer 88(4):579-585(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-940

List Size:
400 ul

List Price:

$495.00

Datasheet
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