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Western blot analysis of AGTR1 in mouse kidney tissue lysate with AGTR1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of AGTR1 in mouse kidney tissue with AGTR1 antibody at 2.5 μg/mL.
Immunofluorescence of AGTR1 in Mouse Kidney cells with AGTR1 antibody at 5 μg/mL.
Immunohistochemistry of AGTR1 in mouse kidney tissue with AGTR1 antibody at 2.5 μg/ml.
Flow Cytometry Validation of AGTR1 in A549 Cells 1X10^6/mL A549 cells were intracellularly stained with 2 μg/mL of AGTR1 antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Western blot analysis of AGTR1 in mouse kidney tissue lysate with AGTR1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

AGTR1 Antibody

CATALOG NUMBER: 5391

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (100%), Rabbit: (100%), Sheep: (94%), Bovine: (94%), Chicken: (81%)
Immunogen:
AGTR1 antibody was raised against a 16 amino acid synthetic peptide from near the center of human AGTR1.
The immunogen is located within amino acids 210 - 260 of AGTR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-2 μg/mL; IHC-P: 2.5 μg/mL; IHC-P/IF: 20 μg/mL;FC: 2 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples; Flow Cytometry in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
AGTR1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AGTR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AGTR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGTR1
Additional Names:
AGTR1 Antibody: AT1, AG2S, AT1B, AT1R, AT1AR, AT1BR, AT2R1, HAT1R, AGTR1B, AGTR1A, AT2R1B, Type-1 angiotensin II receptor, AT1
Protein Accession Number:
EAW78909
Protein Gi Number:
119599315
Ncbi Gene Id Number:
185
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AGTR1 Antibody: Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. AGTR1, the type 1 receptor, is thought to mediate the major cardiovascular effects of angiotensin II and may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. AGTR1 has recently been found to regulate the differentiation of bone marrow-derived monocyte lineage progenitors from hematopoietic stem cells, indicating the diversity of the roles of AGTR1.
Background Reference 1:
Mottl AK, Shoham DA, and North KE. Angiotensin II type 1 receptor polymorphisms and susceptibility to hypertension: A HuGE review. Gen. in Med.2008; 10:560-574.
Background Reference 2:
Miyazaki Y and Ichikawa I. Role of the angiotensin receptor in the development of the mammalian kidney and urinary tract. Comp. Biochem. Physiol. A Mol. Integr. Physiol.2001; 128:89-97.
Background Reference 3:
Tsubakimoto Y, Yamada H, Yokoi H, et al. Bone marrow angiotensin AT1 receptor regulates differentiation of monocyte lineage progenitors from hematopoietic stem cells. Arterioscler. Thromb. Vasc. Biol.2009; 29:1529-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5391

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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