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<strong> Figure 1 WB Validation in Mouse Tissues</strong><br>Loading: 10 μg of lysate Antibodies: AGTR2, 5393, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Rat Heart </strong><br>Loading: 15 μg of lysate Antibodies: AGTR2, 5393, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunofluorescence Validation of AGTR2 in Jurkat Cells/strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat Cells labeling AGTR2 with 5393 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 4 Immunohistochemistry Validation of AGTR2 in Human Lung</strong><br> Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-AGTR2 antibody (5393) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of AGTR2 in Mouse Lung</strong><br> Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-AGTR2 antibody (5393) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunofluorescence Validation of AGTR2 in Rat Lung</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung tissue labeling AGTR2 with 5393 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Figure 1 WB Validation in Mouse Tissues
Loading: 10 μg of lysate Antibodies: AGTR2, 5393, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

AGTR2 Antibody

CATALOG NUMBER: 5393

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, ICC/IF, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%)
Immunogen:
Anti-AGTR2 antibody (5393) was raised against a peptide corresponding to 16 amino acid synthetic peptide near the center of human AGTR2.

The immunogen is located within amino acids 220-270 of AGTR2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, ICC/IF, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P: 1-4 μg/mL; IHC-P/IF: 10-20 μg/mL; ICC/IF: 5-20 μg/mL.

Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1411 - Mouse Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 41 kD

Observed: 41 kD
Purification:
AGTR2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AGTR2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AGTR2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AGTR2
Additional Names:
AGTR2 Antibody: AT2, ATGR2, MRX88, Type-2 angiotensin II receptor, Angiotensin II type-2 receptor, AT2
Protein Accession Number:
AAS45437
Protein Gi Number:
42766665
Ncbi Gene Id Number:
186
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AGTR2 Antibody: Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion that acts through at least two types of receptors, AGTR1 and AGTR2. It is an important effector controlling blood pressure and volume in the cardiovascular system and plays a major role in the development of the mammalian kidney and urinary tract. Like AGTR1, AGTR2 is a seven transmembrane G protein-coupled receptor (GPCR), but AGTR2 does not demonstrate most of the classic features of GPCR signaling. AGTR2 is involved in a wide range of activities, including the induction of neurite outgrowth and the inhibition of cellular proliferation, in addition to the known function of mediation of vasoconstriction.
Background Reference 1:
Mottl AK, Shoham DA, and North KE. Angiotensin II type 1 receptor polymorphisms and susceptibility to hypertension: A HuGE review. Gen. in Med. 2008; 10:560-574.
Background Reference 2:
Miyazaki Y and Ichikawa I. Role of the angiotensin receptor in the development of the mammalian kidney and urinary tract. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2001; 128:89-97.
Background Reference 3:
Laflamme L, Gasparo M, Gallo JM, et al. Angiotensin II induction of neurite outgrowth by AT2 receptors in NG108-15 cells. Effect counteracted by the AT1 receptors. J. Biol. Chem.1996; 271:22729-35.
Background Reference 4:
Stoll M, Steckelings UM, Paul M, et al. The angiotensin AT2-receptor mediates inhibition of cell proliferation in coronary endothelial cells. J. Clin. Invest. 1995; 95:651-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5393

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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