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45-217 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin
45-217 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin

AHI1 Antibody

CATALOG NUMBER: 45-217

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: PTAESEAKVKTC
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave a consistent band at approx 75-80kDa in Human Cerebellum and Frontal Cortex lysates after 0.5ug/ml antibody staining and was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 137kDa according to NP_060121.3Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AHI1
Additional Names:
AHI1, AHI-1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, ORF1, dJ71N10.1, Abelson helper integration site, jouberin, Ahi-1 isoform I, OTTHUMP00000017263, OTTHUMP00000017264, OTTHUMP00000017265, contatins SH3 and WD40 domains, Abelson helper integration site 1
Protein Accession Number:
NP_060121.3,NP_001128304.1,NP_001337433.1
Protein Gi Number:
31542701
Ncbi Gene Id Number:
54806
Background Reference 1:
Jiang X, Hanna Z, Kaouass M, Girard L, Jolicoeur P. Ahi-1, a novel gene encoding a modular protein with WD40-repeat and SH3 domains, is targeted by the Ahi-1 and Mis-2 provirus integrations. J Virol. 2002 Sep;76(18):9046-59.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-217

List Size:
0.1 mg

List Price:

$430.00

Out of stock

Datasheet
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