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Western blot analysis in mouse NIH-3T3(lane 1),T47D cell line(lane 2) lysates (35ug/lane).
AHR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of AHR Antibody with Hela cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse NIH-3T3(lane 1),T47D cell line(lane 2) lysates (35ug/lane).

AHR Antibody

CATALOG NUMBER: 61-965

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This AHR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 555-582 amino acids from the Central region of human AHR.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AHR
Additional Names:
Aryl hydrocarbon receptor, Ah receptor, AhR, Class E basic helix-loop-helix protein 76, bHLHe76, AHR, BHLHE76
Protein Accession Number:
P35869
Protein Gi Number:
3041653
Ncbi Gene Id Number:
196
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AHR is a ligand-activated transcription factorinvolved in the regulation of biological responses to planararomatic hydrocarbons. This receptor has been shown to regulatexenobiotic-metabolizing enzymes such as cytochrome P450. Itsligands included a variety of aromatic hydrocarbons. [provided byRefSeq].
Background Reference 1:
Davila, S., et al. Genes Immun. (2010) In press :
Background Reference 2:
Kalthoff, S., et al. J. Biol. Chem. 285(9):5993-6002(2010)
Background Reference 3:
Hall, J.M., et al. Mol. Endocrinol. 24(2):359-369(2010)
Background Reference 4:
Schroeder, J.C., et al. Biochemistry 49(2):393-400(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-965

List Size:
400 ul

List Price:

$495.00

Datasheet
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