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Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/mL.
Immunofluorescence of AID in Ramos cells with AID antibody at 20 μg/mL.
Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.
Trial Size

AID Antibody

CATALOG NUMBER: 3091

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID.
The immunogen is located within the last 50 amino acids of AID.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1225 - Ramos Cell Lysate
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 25 kDa
Purification:
AID Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AID Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AID antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AICDA
Additional Names:
AID Antibody: AID, ARP2, CDA2, HIGM2, HEL-S-284, AID, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase
Protein Accession Number:
AAM95402
Protein Gi Number:
22297218
Ncbi Gene Id Number:
57379
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AID Antibody: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.
Background Reference 1:
Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
Background Reference 2:
Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.Cell 2000; 102:553-63.
Background Reference 3:
Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
Background Reference 4:
Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3091

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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