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45-220 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-220 (2ug/ml) staining of paraffin embedded Mouse Brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
45-220 (0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

AIF1 Antibody

CATALOG NUMBER: 45-220

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-NKQFLDDPKYSSDED
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5µg/ml. Primary incubation 1 hour at room temperature. IHC: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration: 2-4µg/ml. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.
Specificity:
This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Positive Control 1:
Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIF1
Additional Names:
AIF1, IBA1, allograft inflammatory factor 1 , DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
Protein Accession Number:
NP_001614.3
Protein Gi Number:
14574568
Ncbi Gene Id Number:
199
Background Reference 1:
Yang ZF, Ho DW, Lau CK, Lam CT, Lum CT, Poon RT, Fan ST. Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages. Int Immunol. 2005 Nov;17(11):1391-7. Epub 2005 Sep 12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-220

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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