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Western blot analysis of various lysates using AIF1/IBA1 Mouse mAb at 1:1900 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): SH-SY5Y | Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat thymus tissue using AIF1/IBA1 Mouse mAb at a dilution of 1:50000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human brain tissue using AIF1/IBA1 Mouse mAb followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse brain tissue using AIF1/IBA1 Mouse mAb followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat brain tissue using AIF1/IBA1 Mouse mAb followed by a further incubation with Cy3-conjugated Goat anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using AIF1/IBA1 Mouse mAb at 1:1900 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): SH-SY5Y | Exposure time: 45s.

AIF1/IBA1 Mouse Recombinant Antibody (83-514)

CATALOG NUMBER: 83-514

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-F, IF-P, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-F, IF-P, IHC-P, WB
Application Note:
WB,1:1000 - 1:3000, IF-F,1:100 - 1:400, IF-P,1:100 - 1:400, IHC-P,1:10000 - 1:50000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
17kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Aif1
Additional Names:
G1; Iba1; AIF-1; D17H6S50E
Protein Accession Number:
O70200
Ncbi Gene Id Number:
11629
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables actin filament binding activity and calcium ion binding activity. Involved in several processes, including Rac protein signal transduction; actin filament organization; and ruffle assembly. Acts upstream of or within actin filament bundle assembly. Located in several cellular components, including actin filament; phagocytic cup; and ruffle membrane. Is expressed in adrenal cortex; central nervous system; embryo mesenchyme; and retina. Human ortholog(s) of this gene implicated in type 1 diabetes mellitus. Orthologous to human AIF1 (allograft inflammatory factor 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-514

List Size:
100 uL

List Price:

$434.00

Datasheet
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