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43-651 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.
43-651 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing Mitochondrial staining. The nuclear stain is DA
43-651 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI
43-651 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.

AIFM1 Antibody

CATALOG NUMBER: 43-651

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Cow
Immunogen:
The immunogen for this antibody is: C-DDPNVTKTLRFKQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in lysates of cell line Jurkat (calculated MW of 66.9kDa according to NP_004199.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and NIH3T3 cells. Recommended concentration: 5-10ug/ml.
Specificity:
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1) and isoform 5 (NP_001124319).
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIFM1
Additional Names:
AIFM1, apoptosis-inducing factor, mitochondrion-associated, 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, apoptosis-inducing factor 1, mitochondrial, programmed cell death 8 (apoptosis-inducing factor), striatal apoptosis-inducing factor
Protein Accession Number:
NP_004199.1,NP_665811.1,NP_001124319.1
Protein Gi Number:
22202631
Ncbi Gene Id Number:
9131
Background Reference 1:
Lewis EM, Wilkinson AS, Jackson JS, Mehra R, Varambally S, Chinnaiyan AM, Wilkinson JC.The enzymatic activity of apoptosis-inducing factor supports energy metabolism benefiting the growth and invasiveness of advanced prostate cancer cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-651

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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