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Western blot analysis of AIK using rabbit polyclonal hAIK-H105 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AIK antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of AIK using rabbit polyclonal hAIK-H105 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AIK gene (Lane 2).

AIK Antibody

CATALOG NUMBER: 63-279

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-120 amino acids from the N-terminal region of human AIK.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
46 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AURKA
Additional Names:
Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, AURKA
Protein Accession Number:
O14965
Ncbi Gene Id Number:
6790
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aurora A (AIK) plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.
Background Reference 1:
Kimura, M., et al., J. Biol. Chem. 272(21):13766-13771 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-279

List Size:
400 ul

List Price:

$495.00

Datasheet
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