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Western blot analysis of AIMP1 in (A) human, (B) mouse, and (C) rat brain tissue lysate with AIMP1 antibody at 1 μg/ml.
Immunohistochemistry of AIMP1 in mouse brain tissue with AIMP1 antibody at 5 μg/mL.
Western blot analysis of AIMP1 in (A) human, (B) mouse, and (C) rat brain tissue lysate with AIMP1 antibody at 1 μg/ml.
Trial Size

AIMP1 Antibody

CATALOG NUMBER: 7513

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (73%)
Immunogen:
AIMP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AIMP1.
The immunogen is located within amino acids 240 - 290 of AIMP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
AIMP1 antibody can be used for detection of AIMP1 by Western blot at 1 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
AIMP1 antibody is human, mouse and rat reactive. At least two isoforms of AIMP1 are known to exist; this antibody will recognize both isoforms. AIMP1 antibody is predicted to not cross-react with AIMP2.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 3:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 36 kDa
Purification:
AIMP1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AIMP1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AIMP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AIMP1
Additional Names:
AIMP1 Antibody: p43, HLD3, EMAP2, SCYE1, EMAPII, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, EMAP-2
Protein Accession Number:
NP_001135888
Protein Gi Number:
215490011
Ncbi Gene Id Number:
9255
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AIMP1 (Endothelial monocyte-activating polypeptide II, EMAP2) is a proinflammatory cytokine for monocytes and granulocytes (1). It is specifically induced by apoptosis and is involved in the control of angiogenesis, inflammation, and wound healing (1,2). AIMP1 was identified as one of three auxiliary factors of the mammalian aminoacyl tRNA synthetase (ARS) complex. It binds and facilitates the catalytic reaction of arginyl-tRNA synthetase (2,3). Recent studies show that CD23 plays an essential role in the AIMP1-induced immune response and might be a target in the treatment of inflammatory diseases (4).
Background Reference 1:
Park SG, Jung KH, Lee JS, et al. Precursor of pro-apoptotic cytokine modulates aminoacylation activity of tRNA synthetase. J. Biol. Chem. 1999; 274:16673–6.
Background Reference 2:
Park SG, Shin H, Shin YK, et al. The novel cytokine p43 stimulates dermal fibroblast proliferation and wound repair. Am. J. Pathol. 2005; 166:387–98.
Background Reference 3:
Quevillon S, Agou F, Robinson JC, et al. The p43 component of the mammalian multi-synthetase complex is likely to be the precursor of the endothelial monocyte-activating polypeptide II cytokine. J. Biol. Chem. 1997; 272:32573–9.
Background Reference 4:
Kwon HS, Park MC, Kim DG, et al. Identification of CD23 as a functional receptor for the proinflammatory cytokine AIMP1/p43. J. Cell Sci. 2012; 125:4620-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7513

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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