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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.

AIRE Antibody

CATALOG NUMBER: 27-659

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AIRE.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
AIRE antibody can be used for detection of AIRE by ELISA at 1:312500. AIRE antibody can be used for detection of AIRE by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10409 - Fetal Liver Tissue Lysate
Predicted Molecular Weight:
58 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store AIRE antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
AIRE
Additional Names:
AIRE, APS1, APSI, PGA1, AIRE1, APECED
Protein Accession Number:
NP_000374
Protein Gi Number:
4557291
Ncbi Gene Id Number:
326
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The function of the protein encoded by the AIRE gene is not well defined. However it contains zinc finger motifs suggestive of a transcription factor. The protein (isoform 1) is localized to both the nucleus and cytoplasm. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).
Background Reference 1:
Akiyoshi, H., et al., (2004) J. Biol. Chem. 279 (32), 33984-33991.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-659

List Size:
100 ul

List Price:

$519.00

Datasheet
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