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45-227 (0.3ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-227 staining (0.05ug/ml) of HEK293 cell lysates. Untransfected (Lane 5 and 7) and transfected with Human AIRE (lane 6) or Mouse AIRE (lane 7). Data kindly provided by Prof. Pärt Peterson, University of Tartu, Estonia.
45-227 (0.3ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AIRE Antibody

CATALOG NUMBER: 45-227

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KAKPPKKPESSAEQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in human spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 0.3-1ug/ml.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AIRE
Additional Names:
AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy), autoimmune regulator AIRE
Protein Accession Number:
NP_000374.1
Protein Gi Number:
4557291
Ncbi Gene Id Number:
326
Background Reference 1:
Niki S, Oshikawa K, Mouri Y, Hirota F, Matsushima A, Yano M, Han H, Bando Y, Izumi K, Matsumoto M, Nakayama KI, Kuroda N, Matsumoto M. Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice. J Clin Invest. 2006 May;116(5):1292-301. Epub 2006 Apr 20.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-227

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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