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Western blot analysis of AJAP1 in rat liver tissue lysate with AJAP1 antibody at 1 μg/ml.
Immunocytochemistry of AJAP1 in K562 cells with AJAP1 at 2.5 μg/mL.
Western blot analysis of AJAP1 in rat liver tissue lysate with AJAP1 antibody at 1 μg/ml.
Trial Size

AJAP1 Antibody

CATALOG NUMBER: 8163

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AJAP1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human AJAP1.
The immunogen is located within amino acids 290 - 340 of AJAP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
AJAP1 antibody can be used for detection of AJAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in rat samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
AJAP1 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 48 kDa
Purification:
AJAP1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AJAP1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AJAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AJAP1
Additional Names:
Adherens junctions associated protein 1, Membrane protein shrew-1, MOT8, SHREW1
Protein Accession Number:
NP_061324
Protein Gi Number:
9055278
Ncbi Gene Id Number:
55966
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Adherens Junction Associated Protein 1 (AJAP1) plays a key role in cell adhesion and cell migration (1). It is a transmembrane protein found on the basolateral surface of epithelium and expressed by multiple types of epithelium (1,2). AJAP1 interacts with E-Cadherin and appears to promote the internalization of E-Cadherin upon growth factor stimulation (3). This facilitates the dissolution of adherens junctions with an increase in cell motility (3,4).
Background Reference 1:
Zeng L, Fee BE, Rivas MV, et al. Adherens junctional associated protein-1: a novel 1p36 tumor suppressor candidate in gliomas (Review). Int. J. Oncol. 2014; 45:13-7.
Background Reference 2:
Han L, Zhang KL, Zhang JX, et al. AJAP1 is dysregulated at an early stage of gliomagenesis and suppresses invasion through cytoskeleton reorganization. CNS Neurosci. Ther. 2014; 20:429-37.
Background Reference 3:
Gross JC, Schreiner A, Engels K, et al. E-cadherin surface levels in epithelial growth factor-stimulated cells depend on adherens junction protein shrew-1. Mol. Biol. Cell 2009; 20:3598-607.
Background Reference 4:
Lin N, Di C, Bortoff K, et al. Deletion or epigenetic silencing of AJAP1 on 1p36 in glioblastoma. Mol. Cancer Res. 2012; 10:208-17.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8163

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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