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Western blot analysis of extracts of various cell lines, using AK1 antibody (14-299) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human esophageal cancer using AK1 antibody (14-299) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using AK1 antibody (14-299) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AK1 antibody (14-299). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using AK1 antibody (14-299) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

AK1 Antibody

CATALOG NUMBER: 14-299

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse heart
Positive Control 2:
Mouse skeletal muscle
Predicted Molecular Weight:
Observed: 22kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AK1
Additional Names:
AK1, HTL-S-58j
Ncbi Gene Id Number:
203
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-299

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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