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Western blot analysis of anti-AK1 Pab in Jurkat cell line lysate (35ug/lane)
Western blot analysis of AK1 using rabbit polyclonal AK1 Antibody (S45) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AK1 gene.
Western blot analysis of anti-AK1 Pab in Jurkat cell line lysate (35ug/lane)

AK1 Antibody

CATALOG NUMBER: 63-437

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human AK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AK1
Additional Names:
Adenylate kinase isoenzyme 1 {ECO:0000255, HAMAP-Rule:MF_03171}, AK 1 {ECO:0000255, HAMAP-Rule:MF_03171}, 2743 {ECO:0000255, HAMAP-Rule:MF_03171}, 2746 {ECO:0000255, HAMAP-Rule:MF_03171}, ATP-AMP transphosphorylase 1 {ECO:0000255, HAMAP-Rule:MF_03171}, ATP:AMP phosphotransferase {ECO:0000255, HAMAP-Rule:MF_03171}, Adenylate monophosphate kinase {ECO:0000255, HAMAP-Rule:MF_03171}, Myokinase {ECO:0000255, HAMAP-Rule:MF_03171}, AK1 {ECO:0000255, HAMAP-Rule:MF_03171}
Protein Accession Number:
P00568
Protein Gi Number:
20178288
Ncbi Gene Id Number:
203
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.
Background Reference 1:
Corrons, J.L., et al., Blood 102(1):353-356 (2003).
Background Reference 2:
Toren, A., et al., Br. J. Haematol. 87(2):376-380 (1994).
Background Reference 3:
Zuffardi, O., et al., Hum. Genet. 82(1):17-19 (1989).
Background Reference 4:
Matsuura, S., et al., J. Biol. Chem. 264(17):10148-10155 (1989).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-437

List Size:
400 ul

List Price:

$495.00

Datasheet
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