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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

AK2 Antibody

CATALOG NUMBER: 63-415

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-217 amino acids from the C-terminal region of human AK2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
26 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AK2
Additional Names:
Adenylate kinase 2, mitochondrial {ECO:0000255, HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255, HAMAP-Rule:MF_03168}, 2743 {ECO:0000255, HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255, HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255, HAMAP-Rule:MF_03168}, Adenylate monophosphate kinase {ECO:0000255, HAMAP-Rule:MF_03168}, Adenylate kinase 2, mitochondrial, N-terminally processed {ECO:0000255, HAMAP-Rule:MF_03168}, AK2 {ECO:0000255, HAMAP-Rule:MF_03168}, ADK2
Protein Accession Number:
P54819
Protein Gi Number:
1708596
Ncbi Gene Id Number:
204
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.
Background Reference 1:
Noma, T., et al., Biochim. Biophys. Acta 1395(1):34-39 (1998).
Background Reference 2:
Lee, Y., et al., J. Biochem. 123(1):47-54 (1998).
Background Reference 3:
Lee, Y., et al., Biochem. Mol. Biol. Int. 39(4):833-842 (1996).
Background Reference 4:
Bruns, G.A., et al., Biochem. Genet. 15 (5-6), 477-486 (1977).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-415

List Size:
400 ul

List Price:

$495.00

Datasheet
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