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45-229(0.02ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-229(0.02ug/ml) staining of Human Uterus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AKAP12 Antibody

CATALOG NUMBER: 45-229

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-KQERESAKSELTES
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 200+150kDa bands observed in Human Uterus lysates (calculated MW of 191kDa according to NP_005091.2 and 181kDa according to NP_653080.1). Recommended concentration: 0.02-0.1ug/ml.
Specificity:
This anrtibody is expected to recognize both reported isoforms.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKAP12
Additional Names:
AKAP12, A kinase (PRKA) anchor protein (gravin) 12, GRAVIN, AKAP250, kinase scaffold protein gravin, A kinase (PRKA) anchor protein 12, DKFZp686M0430, DKFZp686O0331
Protein Accession Number:
NP_005091.2,NP_653080.1
Protein Gi Number:
21493022
Ncbi Gene Id Number:
9590
Background Reference 1:
Nauert JB, Klauck TM, Langeberg LK, Scott JD. Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr Biol. 1997 Jan 1;7(1):52-62.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-229

List Size:
0.1 mg

List Price:

$469.00

Out of stock

Datasheet
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