Akap12 Peptide

Application Note:

Akap12 Peptide is used for blocking the activity of Akap12 antibody (catalog number 42-382). The Akap12 antibody ships at 0.5 mg/mL and the Akap12 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.