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45-233 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
45-233 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

AKAP95 Antibody

CATALOG NUMBER: 45-233

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: DQGYGGYGAWSAG-C
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave a band at approx 100kDa in Human Liver lysate after 1ug/ml antibody staining. This band corresponds to the 95kDa band reported in literature (PMID: 10601332). This protein has a calculated MW of 76.1kDa according to NP_005849. Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKAP8
Additional Names:
AKAP8, AKAP95, DKFZp586B1222, A kinase (PRKA) anchor protein 8, A kinase anchor protein 95, A-kinase anchor protein 8, A-kinase anchor protein, 95kDa
Protein Accession Number:
NP_005849.1
Protein Gi Number:
5031579
Ncbi Gene Id Number:
10270
Background Reference 1:
Eide T, Coghlan V, Orstavik S, Holsve C, Solberg R, Skalhegg BS, Lamb NJ, Langeberg L, Fernandez A, Scott JD, Jahnsen T, Tasken K. Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95. Exp Cell Res. 1998 Feb 1;238(2):305-16.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-233

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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