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45-234 (0.03ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing AKR1B10 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with 45-234 in the right panel (mock transfection in first and last lanes).
45-234 (0.03ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AKR1B10 Antibody

CATALOG NUMBER: 45-234

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-QSSHLEDYPFDAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 35kDa band observed in lysates of cell lines HEK293, A549 and HepG2 (calculated MW of 36.0kDa according to NP_064695.2). In transfected HEK293 transiently expressing AKR1B10 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKR1B10
Additional Names:
AKR1B10, aldo-keto reductase family 1, member B10 (aldose reductase) , AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HIS, MGC14103, aldo-keto reductase family 1, member B10, aldo-keto reductase family 1, member B11 (aldose reductase-like), aldose reductase-like 1N: aldose reductase-like peptide, aldose reductase-related protein, small intestine reductase
Protein Accession Number:
NP_064695.2
Protein Gi Number:
20127592
Ncbi Gene Id Number:
57016
Background Reference 1:
Tammali R, Ramana KV, Singhal SS, Awasthi S, Srivastava SK. Aldose reductase regulates growth factor-induced cyclooxygenase-2 expression and prostaglandin E2 production in human colon cancer cells. Cancer Res. 2006 Oct 1;66(19):9705-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-234

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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