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45-235 (0.03ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-235 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-235 (0.03ug/ml) staining of Human Breast lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

AKR1C3 Antibody

CATALOG NUMBER: 45-235

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: CFASHPNYPYSDEY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 35kDa band observed in Human Liver and Human Breast lysates (calculated MW of 36.9kDa according to NP_003730). Recommended concentration: 0.01-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1311 - Human Breast Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKR1C3
Additional Names:
AKR1C3, DD3, HAKRB, HAKRe, HA1753, HSD17B5, hluPGFS, KIAA0119, aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II), prostaglandin F synthase, dihydrodiol dehydrogenase 3, chlordecone reductase homolog, hydroxysteroid (17-beta) dehydrogenase 5, type IIb 3-alpha hydroxysteroid dehydrogenase, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, DDX, aldo-keto reductase family 1, member C3, chlordecone reductase, dihydrodiol dehydrogenase X, type II 3a-hydroxysteroid dehydrogenase, type IIb 3-alpha hydroxysteroid dehydrogenase
Protein Accession Number:
NP_003730.4
Protein Gi Number:
24497583
Ncbi Gene Id Number:
8644
Background Reference 1:
Iczkowski KA, Qiu J, Qian J, Somerville MC, Rittmaster RS, Andriole GL, Bostwick DG. The dual 5-alpha-reductase inhibitor dutasteride induces atrophic changes and decreases relative cancer volume in human prostate. Urology. 2005 Jan;65(1):76-82.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-235

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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