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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line lysates (35ug/lane).
AKR1C3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.

AKR1C3 Antibody

CATALOG NUMBER: 55-966

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This AKR1C3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-135 amino acids from the Central region of human AKR1C3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
37 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AKR1C3
Additional Names:
Aldo-keto reductase family 1 member C3, 1---, 17-beta-hydroxysteroid dehydrogenase type 5, 17-beta-HSD 5, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2, 3-alpha-HSD type 2, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase 3, DD-3, DD3, Dihydrodiol dehydrogenase type I, HA1753, Indanol dehydrogenase, Prostaglandin F synthase, PGFS, Testosterone 17-beta-dehydrogenase 5, Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, AKR1C3, DDH1, HSD17B5, KIAA0119, PGFS
Protein Accession Number:
P42330
Protein Gi Number:
308153646
Ncbi Gene Id Number:
8644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the aldo/keto reductasesuperfamily, which consists of more than 40 known enzymes andproteins. These enzymes catalyze the conversion of aldehydes andketones to their corresponding alcohols by utilizing NADH and/orNADPH as cofactors. The enzymes display overlapping but distinctsubstrate specificity. This enzyme catalyzes the reduction ofprostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and theoxidation of 9alpha,11beta-PGF2 to PGD2. It may play an importantrole in the pathogenesis of allergic diseases such as asthma, andmay also have a role in controlling cell growth and/ordifferentiation. This gene shares high sequence identity with threeother gene members and is clustered with those three genes atchromosome 10p15-p14.
Background Reference 1:
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Background Reference 2:
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Background Reference 3:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 4:
Wang, X., et al. PLoS ONE(8), E11934 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-966

List Size:
400 ul

List Price:

$495.00

Datasheet
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