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Western blot analysis of lysed extracts from HepG2 and HeLa cells using Akt (Ab-308).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-308).
Western blot analysis of lysed extracts from HepG2 and HeLa cells using Akt (Ab-308).

Akt (Ab 308) Antibody

CATALOG NUMBER: 79-295

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Akt (Ab-308) antibody was raised against a peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total Akt protein.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
AKT1
Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, NACP, PARK1, RAC-PK-alpha, Protein kinase B
Protein Accession Number:
NP_001014431.1
Protein Gi Number:
62241013
Ncbi Gene Id Number:
207
Background:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Background Reference 1:
Tremblay F, et al. (2005) Diabetes; 54 (9): 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40): 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6): 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6): 668-77.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-295

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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