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Western blot analysis of lysed extracts from 293 and 3T3 cells using Akt (Ab-473).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-473).
Western blot analysis of lysed extracts from 293 and 3T3 cells using Akt (Ab-473).

Akt (Ab 473) Antibody

CATALOG NUMBER: 79-294

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Akt (Ab-473) antibody was raised against a peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of total Akt protein.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
AKT1
Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, MEK1, PRKMK1, AKT, C-AKT, PKB-alpha
Protein Accession Number:
NP_001014431.1
Protein Gi Number:
62241013
Ncbi Gene Id Number:
207
Background:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Background Reference 1:
Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2): 341-3. Min YH, et al. (2004) Cancer Res; 64 (15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34): 35510-7. Ayala G, et al. (2004) Clin Cancer Res; 10 (19): 6572-8. Lungu AO, et al. (2004) J Biol Chem; 279 (47): 48794-800.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-294

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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