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Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from A549 cells treated with PMA using Phospho-Akt (Ser129) Antibody. The lane on the right is treated with the antigen-specific peptide.

Akt (phospho Ser129) Antibody

CATALOG NUMBER: 79-966

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of Akt only when phosphorylated at serine 129.
Predicted Molecular Weight:
65 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
AKT1
Additional Names:
AKT, AKT1 kinase, kinase Akt1, RAC, RAC-PK-alpha
Protein Accession Number:
NP_001014431.1
Protein Gi Number:
62241013
Ncbi Gene Id Number:
207
Background:
General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
Background Reference 1:
Nguyen le XT, Mitchell BS (2013) Proc Natl Acad Sci U S A 110, 20681-6
Background Reference 2:
Ponce DP, et al. (2011) Mol Cell Biochem 356, 127-32
Background Reference 3:
Siddiqui-Jain A, et al. (2010) Cancer Res 70, 10288-98

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-966

List Size:
0.1 mg

List Price:

$376.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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