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Western Blot: XW-7019 dilution: 1:2,000. Goat anti-13990-HRP dilution: 1:1,000. E coli-derived fusion protein as test antigen.
Western Blot: XW-7019 dilution: 1:2,000. Goat anti-13990-HRP dilution: 1:1,000. E coli-derived fusion protein as test antigen.

AKT1 Antibody

CATALOG NUMBER: XW-7019

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Chicken
Species Reactivity:
Bovine, Frog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Bovine, Frog, Human, Mouse, Rat
Immunogen:
2-200
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
AKT antibody can be used for the detection of AKT by Western blot, may also work for IHC and ICC.
Predicted Molecular Weight:
55.7 kDa (calculated)
Purification:
Antigen affinity-purified
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Phosphate-Buffered Saline. No preservatives added.
Concentration:
1 mg/mL
Storage Conditions:
AKT1 antibody can be stored at 4°C for short term (weeks). Long term storage should be at -20°C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AKT1
Additional Names:
PKB, RACProtein kinase B, PKB, AKT, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA,
Protein Accession Number:
NP_005154.1
Protein Gi Number:
4885061
Ncbi Gene Id Number:
207
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Serine/threonine protein kinase; Murine thymoma viral (v-akt) oncogene homolog-1. AKT1 is a serine-threonine protein kinase. It is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Background Reference 1:
Brunet,A., Bonni,A., Zigmond,M. J., Lin,M. Z., Juo,P., Hu,L. S., Anderson,M. J., Arden,K. C., Blenis,J. and Greenberg,M. E. TITLE Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor JOURNAL Cell 96 (6), 857-868 (1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

XW-7019

List Size:
0.05 mg

List Price:

$626.00

Datasheet
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