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Antibody: 54-321 at 1/1000 dilution<br>Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane<br>Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/60 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
Antibody: 54-321 at 1/1000 dilution<br>Sample: Lane 1: 1ug of PDGF treated NIH-3T3 cell lysate . Lane 2: 500ug of PDGF treated NIH-3T3 cell lysate were incubated with anti-phosop-AKT1 antibody coupled to magnetic beads, Lane 3: Negative control 500ug of PDGF treated NIH-3T3 cell lysate were ncubated with negative control antibody coupled magnetic beads<br>Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution<br>Predicted band size: 56/66 kDa<br>Observed band size: 66 kDa<br>Gel concentration: 4-20%
Antibody: 54-321 at 1/1000 dilution
Sample: NIH-3T3 cell lysate treated with PDGF and without PDGF at 30ug/lane
Secondary HRP conjugated anti-Rabbit Fc at 1/50000 dilution
Predicted band size: 56/60 kDa
Observed band size: 66 kDa
Gel concentration: 4-20%

AKT1 (phospho S473) Antibody [H4]

CATALOG NUMBER: 54-321

Clonality:
Monoclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Also recommended for detection of phosphor-AKT1 in sheep & zebrafish
Immunogen:
Synthetic peptides derived from human AKT1 (RPHFPQFsYSASGTA)
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB, IP dilution (1:1000 – 1: 5000).
Purification:
Protein A purified
Clonality:
Monoclonal
Isotype:
Recombinant Monoclonal Rabbit IgG / light chain kappa
Buffer:
49% PBS, 50% Glycerol, 0.05% BSA, pH 7.2, 0.02% NaN3
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for up to 6 months. Store at -20°C long term
Ncbi Official Symbol:
AKT1
Additional Names:
AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Ncbi Gene Id Number:
207
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

54-321

List Size:
100 ug

List Price:

$515.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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