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Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of AKT1S1 in rat brain tissue with AKT1S1 antibody at 2.5 μg/ml.
Immunofluorescence of AKT1S1 in rat brain tissue with AKT1S1 antibody at 20 μg/ml.
Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

AKT1S1 Antibody

CATALOG NUMBER: 7391

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1.
The immunogen is located within amino acids 190 - 240 of AKT1S1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 28 kDa
Purification:
AKT1S1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AKT1S1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AKT1S1
Additional Names:
AKT1S1 Antibody: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate
Protein Accession Number:
NP_115751
Protein Gi Number:
400153980
Ncbi Gene Id Number:
84335
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).
Background Reference 1:
Saito A, Narasimhan P, Hayashi T, et al. Neuroprotective role of a proline-rich Akt substrate in apoptotic neuronal cell death after stroke: relationships with nerve growth factor. J. Neurosci. 2004; 24:1584-93.
Background Reference 2:
Wiza C, Nascimento EB, and Ouwens DM. Role of PRAS40 in Akt and mTOR signaling in health and disease. Am. J. Physiol. Endocrinol. Metab. 2012; 302:E1453-60.
Background Reference 3:
Wang H, Zhang Q, Zhang L, et al. Insulin-like growth factor-1 induces the phosphrylation of PRAS40 via the PI3K/Akt signaling pathway in PC12 cells. Neurosci. Lett. 516:105-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7391

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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