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Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using Akt2 (Phospho-Ser474).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt2 (Phospho-Ser474).
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with serum (lane 2) using Akt2 (Phospho-Ser474).

Akt2 (phospho Ser474) Antibody

CATALOG NUMBER: 79-157

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Akt2 (Phospho-Ser474) antibody was raised against a peptide sequence around phosphorylation site of serine 474 (Q-F-S (p) -Y-S) derived from Human Akt2.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of Akt2 only when phosphorylated at serine 474.
Predicted Molecular Weight:
60 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
AKT2
Additional Names:
PKBB, PRKBB, HIHGHH, PKBBETA, RAC-BETA, IKKA, TCF16, PKB beta, Protein kinase B, RAC-PK-beta
Protein Accession Number:
NP_001617.1
Protein Gi Number:
4502023
Ncbi Gene Id Number:
208
Background:
General protein kinase capable of phosphorylating several known proteins.
Background Reference 1:
Sun M, et al. (2001) Cancer Res; 61 (16): 5985-91. Yuan ZQ, et al. (2000) Oncogene; 19 (19): 2324-30. Meier R, et al. (1997) J Biol Chem; 272 (48): 30491-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-157

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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