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Western blot analysis of various lysates, using AKT2 Rabbit mAb at 1:3000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using AKT2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using AKT2 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of Jurkat cells using AKT2 Rabbit mAb (Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using AKT2 Rabbit mAb (Red). The cells were counterstained with α-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of 293T cells using AKT2 Rabbit mAb (Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Western blot analysis of various lysates, using AKT2 Rabbit mAb at 1:3000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.

AKT2 Rabbit Recombinant Antibody (82-837)

CATALOG NUMBER: 82-837

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IHC-P, WB
Application Note:
WB,1:3000 - 1:12000, IHC-P,1:200 - 1:2000, IF/ICC,1:200 - 1:400, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
56kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
AKT2
Additional Names:
PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA; AKT2
Protein Accession Number:
P31751
Ncbi Gene Id Number:
208
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-837

List Size:
100 uL

List Price:

$477.00

Datasheet
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