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Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein and human ovary carcinoma tissue lysate .
Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein and human ovary carcinoma tissue lysate .

Akt3 Antibody [3B12F3F10 / 2D2B11B11 / 2D2B11H5]

CATALOG NUMBER: 32-106

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant Akt3 expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1:2000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
Akt3 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AKT3
Additional Names:
PKBG, Protein kinase Akt-3, PKB gamma, MPPH, PKBG, PRKBG, STK-2, PKB-GAMMA, RAC-gamma, RAC-PK-gamma
Protein Accession Number:
Q9Y243
Protein Gi Number:
12643943
Ncbi Gene Id Number:
10000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Akt3 (also designated protein kinase B gamma or v-akt murine thymoma viral oncogene homolog 3)with 479-amino acid protein (about 53 kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt2. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake.Akt3 is not required for the maintenance of normal carbohydrate metabolism but is essential for the attainment of normal organ size. Identifying Akt3 as a selective target in melanoma cells also provides new therapeutic opportunities for patients in the advanced stages of this disease.
Background Reference 1:
Rachael M. Easton, Han Cho, Kristin Roovers. Mol. Cell. Biol., Mar 2005; 25: 1869 – 1878.
Background Reference 2:
Jill M. Stahl, Arati Sharma, Mitchell Cheung. Cancer Res., Oct 2004; 64: 7002–7010.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-106

List Size:
0.1 mL

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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