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<strong>Figure 1 Western Blot Validation in Human Liver </strong><br>Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Liver </strong><br>Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Liver </strong><br>Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of Albumin in Human Liver</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-albumin antibody (5159) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of Albumin in Mouse Liver</strong><br> Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody (5159) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of Albumin in Rat Liver</strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody (5159) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of Albumin in Mouse Liver</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling albumin with 5159 at 20 /mL, followed by goat anti-chicken IgY secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Figure 1 Western Blot Validation in Human Liver
Loading: 10 µg of lysates per lane.Antibodies: Albumin, 5159, 1 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.
Trial Size

Albumin Antibody

CATALOG NUMBER: 5159

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Donkey: (85%), Horse: (85%), Pig: (85%), Rabbit: (85%), Chicken: (80%)
Immunogen:
Albumin antibody was raised against a 13 amino acid synthetic peptide near the center of human Albumin.
The immunogen is located within amino acids 340 - 390 of Albumin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL; IHC-P: 2-10 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples; Immunohistochemistry in human, mouse, and rat samples; . All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 3:
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 69kD

Observed: 69 kD
Isoforms:
Human Albumin has three isoforms, including isoform 1 (609aa, 69kD), isoform 2 (417aa, 47.4kD), and isoform 2 (396aa, 45.2kD). Mouse Albumin has one isoform (608aa, 68.7kD), and rat Albumin has one isoform (608aa, 68.7kD). 5159 can detect human, mouse and rat.
Purification:
Albumin Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
Albumin Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Albumin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALB
Additional Names:
Albumin Antibody: PRO0883, PRO0903, PRO1341, Serum albumin
Protein Accession Number:
P02768
Protein Gi Number:
113576
Ncbi Gene Id Number:
213
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Albumin Antibody: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. It functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The resulting product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. At least two isoforms of Albumin are known to exist.
Background Reference 1:
Rothschild MA, Oratz M, and Schreiber SS. Serum Albumin. Hepatology 1988; 8:385-401.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5159

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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