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45-237 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-237 (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
45-237 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
45-237 (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Aldehyde Reductase Antibody

CATALOG NUMBER: 45-237

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-DAGHPLYPFNDPY
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 36.6kDa according to NP_006057.1 and NP_697021.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
Both reported variants (NP_006057.1and NP_697021.1) represent identical protein
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
AKR1A1
Additional Names:
aldehyde reductase, AKR1A1, aldo-keto reductase family 1, member A1 (aldehyde reductase), ALDR1, ALR, MGC12529, MGC1380, alcohol dehydrogenase, aldo-keto reductase family 1, member A1, DD3, OTTHUMP00000009240, dihydrodiol dehydrogenase 3
Protein Accession Number:
NP_006057.1,NP_697021.1
Protein Gi Number:
5174391
Ncbi Gene Id Number:
10327
Background Reference 1:
Fujii J, Hamaoka R, Matsumoto A, Fujii T, Yamaguchi Y, Egashira M, Miyoshi O, Niikawa N, Taniguchi N. The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome 1p33-->p32. Cytogenet Cell Genet. 1999;84(3-4):230-2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-237

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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