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45-239 (1ug/ml) staining of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing ALDH1A1 and probed with 45-239 (mock transfection in first lane).
45-239 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-239 (1ug/ml) staining of Human Liver Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ALDH1A1 Antibody

CATALOG NUMBER: 45-239

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-RELGEYGFHEYTE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 5ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ALDH1A1
Additional Names:
ALDH1A1, aldehyde dehydrogenase 1 family, member A1 , ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic, retinal dehydrogenase 1
Protein Accession Number:
NP_000680.2
Protein Gi Number:
21361176
Ncbi Gene Id Number:
216
Background Reference 1:
Everts HB, King LE Jr, Sundberg JP, Ong DE. Hair cycle-specific immunolocalization of retinoic acid synthesizing enzymes Aldh1a2 and Aldh1a3 indicate complex regulation. J Invest Dermatol. 2004 Aug;123(2):258-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-239

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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