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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-ALDH4A1 Antibody in HepG2 cell line lysates (35ug/lane).
Western blot analysis of ALDH4A1 using rabbit polyclonal ALDH4A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH4A1 gene.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

ALDH4A1 Antibody

CATALOG NUMBER: 63-261

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ALDH4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-314 amino acids from the Central region of human ALDH4A1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:50~100
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
62 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALDH4A1
Additional Names:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase, ALDH4A1, ALDH4, P5CDH
Protein Accession Number:
P30038
Protein Gi Number:
62511241
Ncbi Gene Id Number:
8659
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.
Background Reference 1:
Yoon,K.A., J. Hum. Genet. 49 (3), 134-140 (2004)
Background Reference 2:
Geraghty,M.T., Hum. Mol. Genet.(9), 1411-1415 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-261

List Size:
400 ul

List Price:

$495.00

Datasheet
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