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42-676 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-676 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-676 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ALDH5A1 Antibody

CATALOG NUMBER: 42-676

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-SQDPAQIWRVAE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human Liver lysates (calculated MW of 58.7kDa according to NP_733936.1). Recommended concentration: 0.3-1ug/ml. Please note that both this product and EB10436 detects a 50kDa in Mouse and Rat Liver lysates (calculated MW of 56.0kDa according to Mouse NP_766120.1).Immunohistochemistry:In paraffin embedded Human Liver shows heavy textured cystoplasm staining in hepatocytes, consistent with mitochondia.. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_733936.1; NP_001071.1). Amino acid numbering in name calculated from NP_733936.1.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ALDH5A1
Additional Names:
ALDH5A1, aldehyde dehydrogenase 5 family, member A1, SSADH, SSDH, NAD(+)-dependent succinic semialdehyde dehydrogenase, aldehyde dehydrogenase 5A1, mitochondrial succinate semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase
Protein Accession Number:
NP_733936.1,NP_001071.1
Protein Gi Number:
25777721
Ncbi Gene Id Number:
7915

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-676

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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