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Western blot analysis of extracts of various cell lines, using ALDH9A1 antibody (23-245) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using ALDH9A1 antibody (23-245) (40x lens).
Immunofluorescence analysis of HeLa cells using ALDH9A1 antibody (23-245) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ALDH9A1 antibody (23-245) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ALDH9A1 Antibody

CATALOG NUMBER: 23-245

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human ALDH9A1
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
22Rv1
Positive Control 3:
NCI-H460
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse kidney
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 54kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ALDH9A1
Additional Names:
ALDH9A1, TMABADH, R-aminobutyraldehyde dehydrogenase, E3, ALDH9, ALDH7, ALDH4, aldehyde dehydrogenase E3 is, aldehyde dehydrogenase 9A1, aldehyde dehydrogenase 9 family, member A1, aldehyde dehydrogenase (NAD+), 4-trimethylaminobutyraldehyde dehydrogenase
Ncbi Gene Id Number:
223
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-245

List Size:
100 uL

List Price:

$436.00

Datasheet
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