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Western blot analysis of lysates from MCF-7, Hela cell line and human skeletal muscle tissue lysate (from left to right), using ALDOA Antibody at 1:1000 at each lane.
Western blot analysis of anti-ALDOA Antibody in mouse liver tissue lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ALDOA antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from MCF-7, Hela cell line and human skeletal muscle tissue lysate (from left to right), using ALDOA Antibody at 1:1000 at each lane.

ALDOA Antibody

CATALOG NUMBER: 61-457

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This ALDOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the C-terminal region of human ALDOA.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ALDOA
Additional Names:
Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDOA, ALDA
Protein Accession Number:
P04075
Protein Gi Number:
113606
Ncbi Gene Id Number:
226
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aldolase A (fructose-bisphosphate aldolase) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.
Background Reference 1:
Gizak,A., Proteins 72 (1), 209-216 (2008) Lu,J., Biochem. Biophys. Res. Commun. 369 (3), 948-952 (2008) Valis,K., Mol. Cell. Biochem. 311 (1-2), 225-231 (2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-457

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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