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43-629 (5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-629 (5ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Alfy / WDFY3 Antibody

CATALOG NUMBER: 43-629

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-SPERSTRTQQKEFQT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 400kDa band observed in Mouse Brain lysates (calculated MW of 395kDa according to NP_055806.2). Data obtained from anonymous customer. The lower molecular weight bands are non-specific. We call for caution when used for other assays than Western blot. Recommended concentration: 4-6ug/ml.
Predicted Molecular Weight:
Approx 400kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Alfy / WDFY3
Additional Names:
WDFY3, WD repeat and FYVE domain containing 3, ALFY, ZFYVE25, WD repeat and FYVE domain-containing protein 3, autophagy-linked FYVE protein
Protein Accession Number:
NP_055806.2
Protein Gi Number:
31317268
Ncbi Gene Id Number:
23001
Background Reference 1:
Yamamoto A, Simonsen A.Alfy-dependent elimination of aggregated proteins by macroautophagy: can there be too much of a good thing?

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-629

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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