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Antibody used in WB on Human NTERA2 at 0.2-1 ug/ml.
Antibody used in WB on Human NTERA2 at 0.2-1 ug/ml.

ALG1 Antibody

CATALOG NUMBER: 26-403

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ALG1 antibody can be used for detection of ALG1 by ELISA at 1:62500. ALG1 antibody can be used for detection of ALG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
NTERA2 Cell Lysate
Predicted Molecular Weight:
52 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ALG1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ALG1
Additional Names:
ALG1, HMAT1, HMT-1, HMT1, MT-1, CDG1K, Mat-1, hMat-1
Protein Accession Number:
NP_061982
Protein Gi Number:
41350216
Ncbi Gene Id Number:
56052
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ALG1 catalyzes the first mannosylation step in the biosynthesis of lipid-linked oligosaccharides. Defects in ALG1 are the cause of congenital disorder of glycosylation type 1K (CDG1K).The biosynthesis of lipid-linked oligosaccharides is highly conserved among eukaryotes and is catalyzed by 14 glycosyltransferases in an ordered stepwise manner. Mannosyltransferase I (MT I) catalyzes the first mannosylation step in this process.
Background Reference 1:
Grubenmann, C.E., (2004) Hum. Mol. Genet. 13 (5), 535-542.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-403

List Size:
100 ul

List Price:

$519.00

Datasheet
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